il 6rα  (Cell Signaling Technology Inc)

Journal: bioRxiv

Article Title: Endothelial ADAM17 Promotes Neutrophil Migration and Pulmonary Microvascular Permeability in ARDS

doi: 10.64898/2026.01.21.700786

Figure Lengend Snippet: (A) Schematic overview of ADAM17 maturation and intracellular transport. (B–D) Schematic representations of TNFR1/2 (B) , IL-6R (C) , and TLR4 (D) signaling pathways. (E) RT-qPCR-based gene expression analysis of inflammatory signaling components in murine lung tissue three hours after LPS inhalation (n = 4/4/10–14/6–8). (F–G) Quantification of TNFR1 (F) and IL-6Rα (G) MFI in lung tissue using LasX (n = 4 mice per group; 10 measurements per image). Data are presented as mean ± SD; Statistical analysis: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, using one-way ANOVA.

Article Snippet: Sections were then incubated with primary antibodies targeting ADAM17 (rabbit anti-ADAM17, polyclonal, Bioss, USA), Ly6G/C (rat anti-Ly6G/C, clone RB6-8C5, Abcam, UK), VE-Cadherin (goat anti-VE-Cadherin, polyclonal, R&D Systems, USA), TNFR1 (mouse anti-TNFR1, clone H-5, Santa Cruz, USA), IL-6Rα (mouse anti-IL-6Rα, clone H-7, Santa Cruz, USA), and vWF (sheep anti-vWF, polyclonal, Abcam, UK) followed by incubation with the corresponding fluorescently labelled secondary antibodies including Alexa Fluor 488 goat anti-rabbit, Alexa Fluor 647 goat anti-rat, Alexa Fluor 488 donkey anti-goat, Alexa Fluor 594 goat anti-mouse, Alexa Fluor 488 donkey anti-mouse and Alexa Fluor 647 donkey anti-sheep.

Techniques: Protein-Protein interactions, Quantitative RT-PCR, Gene Expression